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Journal: Current Issues in Molecular Biology
Article Title: Neuroprotective Effects of Water Extract from Brown Algae Petalonia binghamiae in an Experimental Model of Focal Cerebral Ischemia In Vitro and In Vivo
doi: 10.3390/cimb45100531
Figure Lengend Snippet: wPB inhibits the excitotoxicity-associated apoptosis of HT-22 cells. ( A ) Representative Hoechst33258-stained images and ( B ) the quantitative bar graphs of cells with different treatments. In ( A ), shrunken nuclei and apoptotic bodies are indicated by arrowhead and arrows, respectively. ( C ) Representative dot plot diagrams showing apoptosis-ongoing cells and ( D ) quantitative bar graphs obtained by using AnnexinV-FITC/PI-based FACS. In all graphs, values are presented as the mean ± standard deviation (** p < 0.01 vs. the CTRL cells; # p < 0.05 and ## p < 0.01 vs. the GLU cells; δ p < 0.05 vs. the GLU + wPB10 cells; N.S, not significant between the indicated cells). FITC, fluorescein isothiocyanate; PI, propidium iodide; FACS, fluorescence-activated cell sorting.
Article Snippet: Apoptosis was assessed using flow cytometry and an FITC-conjugated Annexin V apoptosis detection kit (Merck-Millipore, Kenilworth, NJ, USA), in conjunction with an automated fluorescence-activated
Techniques: Staining, Standard Deviation, Fluorescence, FACS